Suppression of DNA damage in human peripheral blood lymphocytes by a juice concentrate: A randomized, double-blind, placebo-controlled trial

Cui X, Jin Y, Singh UP, Chumanevich A, Harmon B, Cavicchia P, Hofsteh Ab, Kotakadi V, Poudyal D, Stroud B, Volate SR, Hurlex TG, Hebert JR, Hofsteh LJ; Mar. 2012


There is increasing evidence that DNA damage in surrogate tissues (i.e., lymphocytes) are elevated in chronic diseases, correlate with the onset of chronic diseases, are elevated in high-risk populations and are lowered by healthy lifestyle factors.  


The aim of this study was to determine whether two formulations of an encapsulated juice powder concentrate could reduce inflammation and attendant DNA damage.  


In this double-blind, placebo-controlled study, 106 participants were randomized to take Fruit and Vegetable Blends (FV; Juice Plus+®), Fruit, Vegetable, and Berry Blends (FVB; Juice Plus+®), or placebo capsules for 60 days. Blood was drawn at baseline and at the end of the study period and was analyzed for markers of inflammation (MCP-1 and RANTES) and DNA damage in peripheral blood lymphocytes (PBL), as measured by the number of DNA strand breaks. Serum micronutrient levels were also analyzed. 


In both intervention groups, beta-carotene, vitamin C, and alpha-tocopherol levels significantly increased after supplementation, while in the placebo group there was no change. In the FV group there was a non-significant trend toward decreased DNA damage, while in the FVB group there was a significant decrease in both single and double strand breaks. In addition, among participants taking the FV and FVB, levels of inflammatory markers were reduced compared to the placebo group. Reductions in inflammation correlated with reductions in DNA damage.  


Overall, our results suggest that the encapsulated FVB blend suppresses PBL DNA damage. This gives a rationale for the use of dietary phytonutrient supplements to suppress inflammation and its biological consequences (e.g. DNA damage).

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